With regards to 'Depth of Field' and without going through the DoF calculation table for a macro lens, It took me foursuccessive shots at f/3.2 to attain complete coverage of the shrimp's eyeball from the front surface to the rear peripheral surface plus another two successive shots to cover the eyestalk anteroposteriorly at the base with a working distance of roughly 6 inches from the tip of the camera lens to the eyes of the shrimp.
Full-stacked macrophotography of a Penaeus vannamei eyes at f/3.2 at the closest allowable working distance from the tip of the camera lens to the frontal surface of the eyes of the shrimp weighing about 25 g
The diameter of the shrimp’s eyes at 25g is approximately 2.6 mm … therefore the DoF of a 105mm f/2.8 macro lens attached to a Nikon D7000 at roughly 6 inches from the shrimp eyes is less than 2.6 mm.
I intentionally set the camera aperture at a shallow depth of field (f/3.2) to create the artistic effect whereby anything in front (foreground) of the eyes of the shrimp such as the antennal plate and antennule and anything at the back (background) of the eyes of the shrimp such as the carapace will be blurry leaving only the eyes (subject) of the shrimp in focus.
Here is the representation of the six successive shots that I took at the closest allowable working distance using a 105mm f/2.8 lens at an aperture opening of f/3.2
Close-range macrophotography by nelson gerundo of the same shrimp (now frozen-thawed) at lateral angle photo-captured out of the water today September 29.2021 (Wednesday) 5 days after it was photographed at a frontal angle.
Each white line represents a single shot fired ... and any parts of the shrimp outside line 1 to line 6 will be blurry because it is outside the depth of field setting of the camera aperture at the closest allowable focusing distance between the camera lens and the targeted part of the shrimp (which in this case is the paired eyes).
Just like this where the antennule and the antennal plate that are in front of the eyes are going to be blurry including the carapace that is behind the eyes.
Leaving only the pair of eyes, the tip of the rostrum, and the base of the paired antennae in focus at the frontal angle of approach.
By mentioning the tip of the rostrum, I meant as indicated by the YELLOW ARROW and for the base of the antennae, I was referring to that part as indicated by the GREEN ARROW.
While this one, on the other hand, was taken at a higher depth of field which I took at an aperture opening of f/16 where every part of the prawn is clear except for the background which I maintained blurry at this aperture setting in close range.
Lest it will look like a point-and-shoot snapshot-like as those taken with a camera phone in automatic mode where everything in the frame (foreground, subject, and background) tends to be always clear.
EXCHANGEABLE IMAGE FILE FORMAT (EXIF) DATA:
https://quotefancy.com/media/wallpaper/3840x2160/6366634-Idowu-Koyenikan-Quote-Many-have-sight-but-few-have-vision.jpg
___________________________
APERTURE OPENING: f/3.2
SHUTTER SPEED: 1/80
ISO: 320
IMAGE FORMAT: Nikon Electronic Format (RAW)
WHITE BALANCE: in automatic mode
FOCAL LENGTH: 10.5 centimeter
PICTURE CONTROL: in standard mode
ACTIVE D-LIGHTING: normal
CENTER WEIGHT AREA: pinpoint focus setting
FLASH: not fired
LENS: 105mm f/2.8
DSLR: Nikon D7000
TRIPOD: Giotto MT 8251
TRIPOD HEAD: GIOTTO MH 1302
POST-PROCESSING SOFTWARE:
Adobe Lightroom
Photoshop
Apple iPhoto
Nelson, awesome piece of art ! What do you reckon is the depth of field ? Regards Keen Wong
Hi Keen,
Thank you for the kind words.
With regards to 'Depth of Field' and without going through the DoF calculation table for a macro lens, It took me four successive shots at f/3.2 to attain complete coverage of the shrimp's eyeball from the front surface to the rear peripheral surface plus another two successive shots to cover the eyestalk anteroposteriorly at the base with a working distance of roughly 6 inches from the tip of the camera lens to the eyes of the shrimp.
The diameter of the shrimp’s eyes at 25g is approximately 2.6 mm … therefore the DoF of a 105mm f/2.8 macro lens attached to a Nikon D7000 at roughly 6 inches from the shrimp eyes is less than 2.6 mm.
Kind regards,
Nelson
Hi Keen
I intentionally set the camera aperture at a shallow depth of field (f/3.2) to create the artistic effect whereby anything in front (foreground) of the eyes of the shrimp such as the antennal plate and antennule and anything at the back (background) of the eyes of the shrimp such as the carapace will be blurry leaving only the eyes (subject) of the shrimp in focus.
https://www.nikonusa.com/en/learn-and-explore/a/tips-and-techniques/understanding-maximum-aperture.html
Kind regards,
Nelson
Hi Keen,
Here is the representation of the six successive shots that I took at the closest allowable working distance using a 105mm f/2.8 lens at an aperture opening of f/3.2
Each white line represents a single shot fired ... and any parts of the shrimp outside line 1 to line 6 will be blurry because it is outside the depth of field setting of the camera aperture at the closest allowable focusing distance between the camera lens and the targeted part of the shrimp (which in this case is the paired eyes).
Kind regards,
Nelson
Hi Keen Wong,
Just like this where the antennule and the antennal plate that are in front of the eyes are going to be blurry including the carapace that is behind the eyes.
Leaving only the pair of eyes, the tip of the rostrum, and the base of the paired antennae in focus at the frontal angle of approach.
http://img.picturequotes.com/2/491/490775/talent-you-have-naturally-skill-is-only-developed-by-hours-and-hours-and-hours-of-beating-on-your-quote-1.jpg
Kind regards,
Nelson
Hi Keen,
By mentioning the tip of the rostrum, I meant as indicated by the YELLOW ARROW and for the base of the antennae, I was referring to that part as indicated by the GREEN ARROW.
http://img.picturequotes.com/2/64/63602/every-science-begins-as-philosophy-and-ends-as-art-quote-1.jpg
Kind regards,
Nelson
Hi Keen,
While this one, on the other hand, was taken at a higher depth of field which I took at an aperture opening of f/16 where every part of the prawn is clear except for the background which I maintained blurry at this aperture setting in close range.
https://pbs.twimg.com/media/EJwNyIIWkAQzWCM.jpg
https://i.pinimg.com/originals/1f/c1/de/1fc1dea7fb96d4a7e5cd9bc34c2df662.jpg
Lest it will look like a point-and-shoot snapshot-like as those taken with a camera phone in automatic mode where everything in the frame (foreground, subject, and background) tends to be always clear.
Kind regards,
Nelson